Observation of stomata by replica method ・ Leaf vein specimen
Commelina communis is generally used for observing stomata. This is because it is easy to peel off the epidermis of the leaves. This time, we will introduce the observation of stomata by the replica method, which allows you to observe even leaves whose epidermis is difficult to remove.
Experiment 1: Observation of stomata by the replica method
you need : Manicure (adhesive), microscope observation tool
Apply nail polish to the leaves. After drying, remove the nail polish and observe it under a microscope. The nail polish is shaped like the epidermis, and although it is transparent and difficult to see, it can be applied to any leaf. It is also suitable for observing the epidermis of parts other than the leaves.
Observation of pores such as pine
you need : Pine leaves and cypress leaves, binocular stereomicroscope
Conifers do not have stomatal guard cells. For that reason, it is whitened by the wax component. This is called the stomatal zone. The lower right photo is the pine pores. They are lined up neatly. The stomata of pine trees that grow in areas with severe air pollution are closed in black. It is used for the theme of independent research.
Experiment 2: Preparation of vein specimen
you need : Hard leaves such as holly, baking soda, pot, toothbrush
Heat 20 g of baking soda until no gas is generated. Add 200g of water and simmer the leaves. It takes about an hour to use baking soda, but it is easy to treat the chemicals after the experiment. If the amount of water decreases during the experiment, add it.
Remove the mesophyll with a toothbrush in water, leaving only the veins. Laminate the resulting specimen.
It takes time, but I think it is significant for children to have my own specimens. I will treat it carefully and I am sure that my interest in science will expand.